Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay.
نویسندگان
چکیده
We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
منابع مشابه
Technical note: direct genotyping of the double-muscling locus (mh) in Piedmontese and Belgian Blue cattle by fluorescent PCR.
A simple PCR-based allele detection system has been developed to assist in the management of the two most prevalent double-muscled (mh) breeds in the U.S. Application of this assay will permit the implementation of structured mating systems dependent on precise genotypes at the mh locus. The genetic assay uses standard fluorescent genotyping technology and relies on the unique nucleotide compos...
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Myostatin or growth and differentiation factor 8 (GDF8), has been known as the factor causing double muscling phenotypes in which a series of mutations make the myostatin protein inactive, hence disabling it to regulate the deposition of muscle fibre. This phenotype happens with high frequency in a breed of sheep known as the Texel. Quantitative trait loci (QTL) studies show that a portion of t...
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A visibly distinct muscular hypertrophy (mh), commonly known as double muscling, occurs with high frequency in the Belgian Blue and Piedmontese cattle breeds. The autosomal recessive mh locus causing double-muscling condition in these cattle maps to bovine chromosome 2 within the same interval as myostatin, a member of the TGF-beta superfamily of genes. Because targeted disruption of myostatin ...
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متن کاملMolecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses.
Excessive muscling in double-muscled cattle arises from mutations in the myostatin gene, but the role of myostatin in normal muscle development is unclear. The aim of this study was to measure the temporal relationship of myostatin and myogenic regulatory factors during muscle development in normal (NM)- and double-muscled (DM) cattle to determine the timing and possible targets of myostatin ac...
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عنوان ژورنال:
- Animal genetics
دوره 31 6 شماره
صفحات -
تاریخ انتشار 2000